Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 549962
Title Application of a partial cell recycling chemostat for continuous production of aroma compounds at near-zero growth rates
Author(s) Mastrigt, Oscar van; Egas, Reinier A.; Lillevang, Søren K.; Abee, Tjakko; Smid, Eddy J.
Source BMC Research Notes 12 (2019)1. - ISSN 1756-0500
DOI https://doi.org/10.1186/s13104-019-4213-4
Department(s) Food Microbiology
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2019
Keyword(s) Continuous cultivation - Fermentation - Lactic acid bacteria - Maintenance - Metabolomics - Retentostat - VOC
Abstract

Objective: The partial cell recycling chemostat is a modification of the chemostat in which cells are partially recycled towards the bioreactor. This allows using dilution rates higher than the maximum growth rate resulting in higher biomass concentrations and increased process rates. In this study, we demonstrate with a single observation that this system can also be used to study microorganisms at near-zero growth rates and as production system for compounds specific for slow growth, such as those typical for ripened cheese. Results: Lactococcus lactis FM03-V2 was cultivated at growth rates between 0.0025 and 0.025 h -1 . Detailed analysis of produced aroma compounds revealed that levels of particular compounds were clearly affected by the growth rate within the studied range demonstrating that we can steer the aroma production by controlling the growth rate. With this approach, we also experimentally validated that the maintenance coefficient of this dairy strain decreased at near-zero growth rates (6.4-fold). An exponentially decreasing maintenance coefficient was included in the growth model, enabling accurate prediction of biomass accumulation in the partial cell recycling chemostat. This study demonstrates the potential of partial cell recycling chemostat both as aroma production system at near-zero growth rates and as unique research tool.

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