Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 550065
Title Understanding mild cell disintegration of microalgae in bead mills for the release of biomolecules
Author(s) Suarez Garcia, E.; Lo, C.; Eppink, M.H.M.; Wijffels, R.H.; Berg, C. van den
Source Chemical Engineering Science 203 (2019). - ISSN 0009-2509 - p. 380 - 390.
Department(s) Bioprocess Engineering
FBEZ, Financiele Administratie
Publication type Refereed Article in a scientific journal
Publication year 2019
Keyword(s) Bead mill - Biorefinery - Disintegration - Energy efficient - Microalgae - Selective release

Cell disintegration is, in general, the first step in the biorefinery of algae, since it allows the release of biomolecules of interest from the cells into the bulk medium. For high-value commercial applications, the disintegration process must be selective, energy efficient and mild. Developing a process with such features would demand extensive experimental effort. In the present study, we attempt to provide a tool for developing an efficient disintegration process via bead milling, by proposing a modelling strategy that allows the prediction of the kinetics of cell disintegration while having as input not only process parameters but also strain-specific parameters like cell size and cell-wall strength. The model was validated for two different algal strains (Tetraselmis suecica and Chlorella vulgaris), at various values of bead size (0.3–1 mm) and bead fillings (2.5–75%) and at two different scales of 80 and 500 mL. Since the kinetics of disintegration is proportional to the kinetics of release of biomolecules, the model can be further used for scale-up studies and to establish a window of operation to selectively target cells or metabolites of interest. Furthermore, the energy consumption in the mill was evaluated and it was found that operating at high bead fillings (>65%) is crucial to ensure an energy efficient process.

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