Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 550178
Title Characterisation of a novel virus infecting orchids of the genus Pleione
Author(s) Komínek, Petr; Massart, Sébastien; Pham, Khanh; Leeuwen, Paul van; Komínková, Marcela
Source Virus Research 261 (2019). - ISSN 0168-1702 - p. 56 - 59.
Department(s) GTB Gewasgez. Bodem en Water
WPR GTB Bloembollen
Publication type Refereed Article in a scientific journal
Publication year 2019
Keyword(s) Aphid transmission - Electron microscopy - High-throughput sequencing - Next generation sequencing - Pleione flower breaking virus - Potyvirus

Plants of the genus Pleione, originating from hobby growers in the Netherlands and in the Czech Republic, were conspicuous for viral infection, showing symptoms of leaf mosaic or flower breaking. Using Sanger and high throughput sequencing, the full genome sequence of a novel potyvirus was obtained from sequencing data. The genome sequence was annotated and compared to the genome of other potyviruses. The virus was experimentally transmitted by aphids into Pleione and Chenopodium quinoa plants. The name Pleione flower breaking virus (PlFBV) was suggested for the new virus. The presence of the virus was confirmed using RT-PCR, with newly designed primers targeting this new species. The incidence of the virus was contrasted between both countries and might have been influenced by the growth conditions and the exposure of the plants to aphids.

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