Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 550720
Title Alcohol acetyl transferases for alkyl alkanoate production
Author(s) Kruis, A.J.; Levisson, M.; Mars, A.E.; Kengen, S.W.M.; Oost, J. van der; Sanders, J.P.M.; Weusthuis, R.A.
Source Octrooinummer: WO2018099719, verleend: 2018-06-08.
Department(s) Bioprocess Engineering
Laboratory of Plant Physiology
VLAG
BBP Bioconversion
Microbiology
Biobased Products
Publication type Patent
Publication year 2018
Abstract The present invention is directed to a process for the production of alkyl alkanoate wherein a polypeptide that has alcohol acetyl transferase activity (AAT) and has in its three-dimensional structure an alpha-beta hydrolase fold and an active site comprising a serine, histidine, and optionally aspartic acid is, used to convert: - C3-C10 alkanol and acetyl coenzyme A or, - C1-C10 alkanol with C3-C10 acyl coenzyme A, or - Methanol with acetyl coenzyme A, into an alkyl alkanoate. The process is further directed to the use of a recombinant expression vector or plasmid or host cell comprising the nucleotide sequence encoding the polypeptide. The polypeptides according to the invention are genes isolated from Kluyveromyces marxianus, Kluyveromyces lactis, Wickerhamomyces ciferrii, Cyberlindnera jadinii, Hanseniaspora uvarum, Eremothecium cymbalarie and Saccharomyces cerevisiae.
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