Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 551029
Title Toward developing a yeast cell factory for the production of prenylated flavonoids
Author(s) Levisson, Mark; Araya-Cloutier, Carla; Bruijn, Wouter J.C. De; Heide, Menno Van Der; Salvador López, José Manuel; Daran, Jean Marc; Vincken, Jean Paul; Beekwilder, Jules
Source Journal of Agricultural and Food Chemistry (2019). - ISSN 0021-8561
DOI https://doi.org/10.1021/acs.jafc.9b01367
Department(s) Laboratory of Plant Physiology
Food Chemistry
VLAG
BIOS Applied Metabolic Systems
Publication type Refereed Article in a scientific journal
Publication year 2019
Keyword(s) 8-prenylnaringenin - de novo - metabolic engineering - naringenin - prenylated flavonoids - Saccharomyces cerevisiae
Abstract

Prenylated flavonoids possess a wide variety of biological activities, including estrogenic, antioxidant, antimicrobial, and anticancer activities. Hence, they have potential applications in food products, medicines, or supplements with health-promoting activities. However, the low abundance of prenylated flavonoids in nature is limiting their exploitation. Therefore, we investigated the prospect of producing prenylated flavonoids in the yeast Saccharomyces cerevisiae. As a proof of concept, we focused on the production of the potent phytoestrogen 8-prenylnaringenin. Introduction of the flavonoid prenyltransferase SfFPT from Sophora flavescens in naringenin-producing yeast strains resulted in de novo production of 8-prenylnaringenin. We generated several strains with increased production of the intermediate precursor naringenin, which finally resulted in a production of 0.12 mg L -1 (0.35 μM) 8-prenylnaringenin under shake flask conditions. A number of bottlenecks in prenylated flavonoid production were identified and are discussed.

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