Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 551142
Title Risk-Based Bioengineering Strategies for Reliable Bacterial Vaccine Production
Author(s) Kamminga, Tjerko; Slagman, Simen Jan; Martins dos Santos, Vitor A.P.; Bijlsma, Jetta J.E.; Schaap, Peter J.
Source Trends in Biotechnology 37 (2019)8. - ISSN 0167-7799 - p. 805 - 816.
DOI https://doi.org/10.1016/j.tibtech.2019.03.005
Department(s) Systems and Synthetic Biology
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2019
Keyword(s) bacterial vaccines - constraint-based - genome-scale metabolic models - process analytical technology - risk-based process design
Abstract

Design of a reliable process for bacterial antigen production requires understanding of and control over critical process parameters. Current methods for process design use extensive screening experiments for determining ranges of critical process parameters yet fail to give clear insights into how they influence antigen potency. To address this gap, we propose to apply constraint-based, genome-scale metabolic models to reduce the need of experimental screening for strain selection and to optimize strains based on model driven iterative Design–Build–Test–Learn (DBTL) cycles. Application of these systematic methods has not only increased the understanding of how metabolic network properties influence antigen potency, but also allows identification of novel critical process parameters that need to be controlled to achieve high process reliability.

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