Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 551190
Title Detection and profiling of diarrheic marine biotoxins in shellfish by mRNA analysis of exposed Caco-2 cells using qRT-PCR and multiplex magnetic bead-based assays
Author(s) Bodero, Marcia; Bovee, Toine F.H.; Portier, Liza; Hendriksen, Peter J.M.
Source Altex 36 (2019)2. - ISSN 1868-596X - p. 203 - 214.
Department(s) BU Authenticity & Bioassays
BU Toxicology, Novel Foods & Agrochains
Publication type Refereed Article in a scientific journal
Publication year 2019

The mouse bioassay for the detection of marine biotoxins in shellfish products is 40 years old and still in use. A full ban or total replacement of this in vivo test has been postponed because of the fear that current chemical-based detection methods could miss a new emerging toxin. In order to fully replace the mouse bioassay, more efforts are needed in the search for functional assays with specific endpoints. Gene expression elicited by diarrheic shellfish poisons (DSP) in Caco-2 cells allowed us to determine three “DSP profiles”, i.e., OA/DTX, AZA-YTX, and PTX profiles. Twelve marker genes were selected to represent the three profiles. qRT-PCR is relatively cheap and easy, and although its multiplex capacity is limited to 5 genes, this was sufficient to show the three expected profiles. The use of the multiplex magnetic bead-based assay was an even better alternative, allowing the detection of all 12 selected marker genes and 2 reference genes, and resulting in clear profiles with for some genes even higher induction factors than obtained by qRT-PCR. When analyzing blank and contaminated shellfish samples with the multiplex magnetic bead-based assay, the contaminated samples could easily be distinguished from the blank samples, and showed the expected profiles. This work is one step further towards the final replacement of the mouse bioassay. We suggest to combine the neuro-2a bioassay for screening with detection by analytical chemical analyses and with the multiplex magnetic bead-based assay for confirmation of known and unknown toxins.

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