uantitative data on rhizodeposition under ecologically realistic conditions are scarce. Yet they are necessary to understand various aspects of soil organic matter dynamics. To evaluate the use of i4C pulse-labelling for rhizosphere carbon budget estimations and to develop a standard labelling procedure, the dynamics of 14C partitioning and factors affecting the representativity of the assimilated r4C for the average daily assimilation were investigated. Field-grown spring wheat plants were pulselabelled with i4C at five different development stages between elongation and dough ripening. Allocation of 14C in shoot tissue and soil-root respiration was complete by day 19 after labelling. The distribution of net fixed i4C was not affected by the time of day when labelling was performed. Therefore, net assimilated i4C was representative for the average daily net assimilation. The proportion of net fixed i4C recovered in the shoot increased from 61% at elongation to 85% at dough ripening. In the roots this proportion decreased from 15 to 2% and in soil-root respiration from 14 to 7%, while in the soil organic C the percentage did not change with the development stage. 14C in roots and soil organic C decreased exponentially with depth. We can conclude that i4C pulse-labelling of wheat plants with an allocation period of about 3 weeks is a satisfactory method to estimate assimilate distribution at different development stages.
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