Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 551620
Title Multiplex genome editing of microorganisms using CRISPR-Cas
Author(s) Adiego-Pérez, Belén; Randazzo, Paola; Daran, Jean Marc; Verwaal, René; Roubos, Johannes A.; Daran-Lapujade, Pascale; Oost, John van der
Source FEMS Microbiology Letters 366 (2019)8. - ISSN 0378-1097
DOI https://doi.org/10.1093/femsle/fnz086
Department(s) BacGen
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2019
Keyword(s) Cas12a - Cas9 - cell factories - CRISPR-Cas - genome editing - multiplex
Abstract

Microbial production of chemical compounds often requires highly engineered microbial cell factories. During the last years, CRISPR-Cas nucleases have been repurposed as powerful tools for genome editing. Here, we briefly review the most frequently used CRISPR-Cas tools and describe some of their applications. We describe the progress made with respect to CRISPR-based multiplex genome editing of industrial bacteria and eukaryotic microorganisms. We also review the state of the art in terms of gene expression regulation using CRISPRi and CRISPRa. Finally, we summarize the pillars for efficient multiplexed genome editing and present our view on future developments and applications of CRISPR-Cas tools for multiplex genome editing.

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