Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 551782
Title Characterization of heterotrophic growth and sesquiterpene production by Rhodobacter sphaeroides on a defined medium
Author(s) Orsi, Enrico; Folch, Pauline L.; Monje-López, Vicente T.; Fernhout, Bas M.; Turcato, Alessandro; Kengen, Servé W.M.; Eggink, Gerrit; Weusthuis, Ruud A.
Source Journal of Industrial Microbiology and Biotechnology 46 (2019)8. - ISSN 1367-5435 - p. 1179 - 1190.
Department(s) Bioprocess Engineering
Publication type Refereed Article in a scientific journal
Publication year 2019
Keyword(s) Amorphadiene - MEP - Mevalonate - PHB - Rhodobacter sphaeroides

Rhodobacter sphaeroides is a metabolically versatile bacterium capable of producing terpenes natively. Surprisingly, terpene biosynthesis in this species has always been investigated in complex media, with unknown compounds possibly acting as carbon and nitrogen sources. Here, a defined medium was adapted for R. sphaeroides dark heterotrophic growth, and was used to investigate the conversion of different organic substrates into the reporter terpene amorphadiene. The amorphadiene synthase was cloned in R. sphaeroides, allowing its biosynthesis via the native 2-methyl-d-erythritol-4-phosphate (MEP) pathway and, additionally, via a heterologous mevalonate one. The latter condition increased titers up to eightfold. Consequently, better yields and productivities to previously reported complex media cultivations were achieved. Productivity was further investigated under different cultivation conditions, including nitrogen and oxygen availability. This novel cultivation setup provided useful insight into the understanding of terpene biosynthesis in R. sphaeroides, allowing to better comprehend its dynamics and regulation during chemoheterotrophic cultivation.

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