Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 551803
Title Genetic and molecular analysis of trichome development in Arabis alpina
Author(s) Chopra, Divykriti; Mapar, Mona; Stephan, Lisa; Albani, Maria C.; Deneer, Anna; Coupland, George; Willing, Eva Maria; Schellmann, Swen; Schneeberger, Korbinian; Fleck, Christian; Schrader, Andrea; Hülskamp, Martin
Source Proceedings of the National Academy of Sciences of the United States of America 116 (2019)24. - ISSN 0027-8424 - p. 12078 - 12083.
DOI https://doi.org/10.1073/pnas.1819440116
Department(s) Mathematical and Statistical Methods - Biometris
Systems and Synthetic Biology
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2019
Keyword(s) Arabis alpina - Genetic analysis - Trichomes
Abstract

The genetic and molecular analysis of trichome development in Arabidopsis thaliana has generated a detailed knowledge about the underlying regulatory genes and networks. However, how rapidly these mechanisms diverge during evolution is unknown. To address this problem, we used an unbiased forward genetic approach to identify most genes involved in trichome development in the related crucifer species Arabis alpina. In general, we found most trichome mutant classes known in A. thaliana. We identified orthologous genes of the relevant A. thaliana genes by sequence similarity and synteny and sequenced candidate genes in the A. alpina mutants. While in most cases we found a highly similar gene-phenotype relationship as known from Arabidopsis, there were also striking differences in the regulation of trichome patterning, differentiation, and morphogenesis. Our analysis of trichome patterning suggests that the formation of two classes of trichomes is regulated differentially by the homeodomain transcription factor AaGL2. Moreover, we show that overexpression of the GL3 basic helix–loop–helix transcription factor in A. alpina leads to the opposite phenotype as described in A. thaliana. Mathematical modeling helps to explain how this nonintuitive behavior can be explained by different ratios of GL3 and GL1 in the two species.

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