Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 551863
Title Optical trapping in plant cells
Author(s) Ketelaar, Tijs; Ruijter, Norbert de; Niehren, Stefan
Source In: Methods in Molecular Biology / Cvrcková, F., Zárský, V., Hertfordshire : Springer (Methods in Molecular Biology ) - ISBN 9781493994687 - p. 231 - 238.
DOI https://doi.org/10.1007/978-1-4939-9469-4_15
Department(s) EPS
Laboratory of Cell Biology
Publication type Peer reviewed book chapter
Publication year 2019
Keyword(s) Confocal microscope - Cytoskeleton - Endomembrane system - Noninvasive manipulation - Optical trap - Optical tweezers - Plant cell
Abstract

Optical tweezers allow for noninvasive manipulation of subcellular compartments to study their physical interactions and attachments. By measuring (delay of) displacements, (semi)quantitative force measurements within a living cell can be performed. In this chapter, we provide practical tips for setting up such experiments paying special attention to the technical considerations for integrating optical tweezers into a confocal microscope. Next, we describe experimental approaches we have taken to trap intracellular structures in plant cells.

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