Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 552040
Title Live cell imaging of meiosis in Arabidopsis thaliana
Author(s) Prusicki, Maria A.; Keizer, Emma M.; Rosmalen, Rik P. van; Komaki, Shinichiro; Seifert, Felix; Müller, Katja; Wijnker, Erik; Fleck, Christian; Schnittger, Arp
Source eLife 8 (2019). - ISSN 2050-084X
DOI https://doi.org/10.7554/eLife.42834
Department(s) Mathematical and Statistical Methods - Biometris
Systems and Synthetic Biology
EPS
Laboratory of Genetics
Publication type Refereed Article in a scientific journal
Publication year 2019
Keyword(s) A. thaliana - cell biology - cyclin - development - meiosis - phragmoplast - plant biology - reproduction - spindle
Abstract

To follow the dynamics of meiosis in the model plant Arabidopsis, we have established a live cell imaging setup to observe male meiocytes. Our method is based on the concomitant visualization of microtubules (MTs) and a meiotic cohesin subunit that allows following five cellular parameters: cell shape, MT array, nucleus position, nucleolus position, and chromatin condensation. We find that the states of these parameters are not randomly associated and identify 11 cellular states, referred to as landmarks, which occur much more frequently than closely related ones, indicating that they are convergence points during meiotic progression. As a first application of our system, we revisited a previously identified mutant in the meiotic A-type cyclin TARDY ASYNCHRONOUS MEIOSIS (TAM). Our imaging system enabled us to reveal both qualitatively and quantitatively altered landmarks in tam, foremost the formation of previously not recognized ectopic spindle- or phragmoplast-like structures that arise without attachment to chromosomes.

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