|Title||The Cdk1/Cdk2 homolog CDKA;1 controls the recombination landscape in Arabidopsis|
|Author(s)||Wijnker, Erik; Harashima, Hirofumi; Müller, Katja; Parra-Nuñez, Pablo; Bastiaan de Snoo, C.; Belt, Jose van de; Dissmeyer, Nico; Bayer, Martin; Pradillo, Monica; Schnittger, Arp|
|Source||Proceedings of the National Academy of Sciences of the United States of America 116 (2019)25. - ISSN 0027-8424 - p. 12534 - 12539.|
Laboratory of Genetics
|Publication type||Refereed Article in a scientific journal|
|Keyword(s)||Class - Cross-over interference - Crossovers - Cyclin-dependent kinase - Meiosis - Meiotic recombination|
Little is known how patterns of cross-over (CO) numbers and distribution during meiosis are established. Here, we reveal that cyclin-dependent kinase A;1 (CDKA;1), the homolog of human Cdk1 and Cdk2, is a major regulator of meiotic recombination in Arabidopsis. Arabidopsis plants with reduced CDKA;1 activity experienced a decrease of class I COs, especially lowering recombination rates in centromere-proximal regions. Interestingly, this reduction of type I CO did not affect CO assurance, a mechanism by which each chromosome receives at least one CO, resulting in all chromosomes exhibiting similar genetic lengths in weak loss-of-function cdka;1 mutants. Conversely, an increase of CDKA;1 activity resulted in elevated recombination frequencies. Thus, modulation of CDKA;1 kinase activity affects the number and placement of COs along the chromosome axis in a dose-dependent manner.