|Title||Overexpression of delta-12 desaturase in the yeast Schwanniomyces occidentalis enhances the production of linoleic acid|
|Author(s)||Lamers, Dennis; Visscher, Bram; Weusthuis, Ruud A.; Francke, Christof; Wijffels, René H.; Lokman, Christien|
|Source||Bioresource Technology 289 (2019). - ISSN 0960-8524|
|Publication type||Refereed Article in a scientific journal|
|Keyword(s)||FAD2 - Fatty acid desaturase - Linoleic acid - Schwanniomyces occidentalis|
The oleaginous yeast Schwanniomyces occidentalis was previously isolated because of its excellent suitability to convert lignocellulosic hydrolysates into triacyl glycerides: it is able to use a broad range of sugars and is able to tolerate high concentrations of lignocellulosic hydrolysate inhibitors. Compared to other oleaginous yeasts S. occidentalis however produces a low content of unsaturated fatty acids. We show here that the linoleic acid content can be significantly improved by (over)expression Δ12-desaturases derived from S. occidentalis and Fusarium moniliforme. Expression was stable for the homologous expression but decreased during heterologous expression. Both homologous and heterologous expression of mCherry-Δ12-desaturase led to a 4-fold increase in linoleic acid from 0.02 g/g biomass to 0.08 g/g biomass resulting in the production of 2.23 g/L and 2.05 g/L of linoleic acid.