Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 552071
Title Disease-free monoculture farming by fungus-growing termites
Author(s) Otani, Saria; Challinor, Victoria L.; Kreuzenbeck, Nina B.; Kildgaard, Sara; Krath Christensen, Søren; Larsen, Louise Lee Munk; Aanen, Duur K.; Rasmussen, Silas Anselm; Beemelmanns, Christine; Poulsen, Michael
Source Scientific Reports 9 (2019)1. - ISSN 2045-2322
DOI https://doi.org/10.1038/s41598-019-45364-z
Department(s) PE&RC
Laboratory of Genetics
Publication type Refereed Article in a scientific journal
Publication year 2019
Abstract

Fungus-growing termites engage in an obligate mutualistic relationship with Termitomyces fungi, which they maintain in monocultures on specialised fungus comb structures, without apparent problems with infectious diseases. While other fungi have been reported in the symbiosis, detailed comb fungal community analyses have been lacking. Here we use culture-dependent and -independent methods to characterise fungus comb mycobiotas from three fungus-growing termite species (two genera). Internal Transcribed Spacer (ITS) gene analyses using 454 pyrosequencing and Illumina MiSeq showed that non-Termitomyces fungi were essentially absent in fungus combs, and that Termitomyces fungal crops are maintained in monocultures as heterokaryons with two or three abundant ITS variants in a single fungal strain. To explore whether the essential absence of other fungi within fungus combs is potentially due to the production of antifungal metabolites by Termitomyces or comb bacteria, we performed in vitro assays and found that both Termitomyces and chemical extracts of fungus comb material can inhibit potential fungal antagonists. Chemical analyses of fungus comb material point to a highly complex metabolome, including compounds with the potential to play roles in mediating these contaminant-free farming conditions in the termite symbiosis.

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