Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 552367
Title The cysteine-rich region of a baculovirus VP91 protein contributes to the morphogenesis of occlusion bodies
Author(s) Zhou, Fengqiao; Kuang, Wenhua; Wang, Xi; Hou, Dianhai; Huang, Huachao; Sun, Xiulian; Deng, Fei; Wang, Hualin; Oers, Monqiue M. van; Wang, Manli; Hu, Zhihong
Source Virology 535 (2019). - ISSN 0042-6822 - p. 144 - 153.
DOI https://doi.org/10.1016/j.virol.2019.06.016
Department(s) Laboratory of Virology
EPS
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2019
Keyword(s) Cysteine-rich region - Disulfide bond - HA76 - Occlusion body (OB) - Oral infection - P33 - Per os infectivity factor (PIF) - PIF8 - Polyhedra - VP91
Abstract

The baculovirus core gene vp91 has been reported to be essential for nucleocapsid assembly and oral infection. Here, we studied the function of vp91 by analyzing its homologue, ha76, in Helicoverpa armigera nucleopolyhedrovirus (HearNPV). HA76 was expressed at the late stage of HearNPV infection; deletion of ha76 showed that the gene is required for budded virus production. A series of recombinants with truncated ha76 was constructed and analyzed in vitro and in vivo. The results showed that the region encoding the C-terminus of HA76 was essential for nucleocapsid assembly, whereas the N-terminal cysteine-rich region was responsible for oral infection. Electron microscope analyses further showed that the cysteine-rich region contributed to morphogenesis of occlusion bodies (OBs), with amino acids 136–223 of HA76 being critical for this function. The results revealed a novel function of VP91 and suggested that the impact on OB morphogenesis is partially related to oral infectivity.

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