Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 552752
Title Astin C Production by the Endophytic Fungus Cyanodermella asteris in Planktonic and Immobilized Culture Conditions
Author(s) Vassaux, Antoine; Tarayre, Cédric; Arguëlles-Arias, Anthony; Compère, Philippe; Delvigne, Frank; Fickers, Patrick; Jahn, Linda; Lang, Alexander; Leclère, Valérie; Ludwig-Müller, Jutta; Ongena, Marc; Schafhauser, Thomas; Telek, Samuel; Théatre, Ariane; Berkel, Willem J.H. van; Vandenbol, Micheline; Pée, Karl Heinz van; Willems, Luc; Wohlleben, Wolfgang; Jacques, Philippe
Source Biotechnology Journal 14 (2019)8. - ISSN 1860-6768
Department(s) VLAG
Publication type Refereed Article in a scientific journal
Publication year 2019
Keyword(s) astin C - biofilms - Cyanodermella asteris - immobilized-cell cultures - secondary metabolites

The fungal endophyte Cyanodermella asteris (C. asteris) has been recently isolated from the medicinal plant Aster tataricus (A. tataricus). This fungus produces astin C, a cyclic pentapeptide with anticancer and anti-inflammatory properties. The production of this secondary metabolite is compared in immobilized and planktonic conditions. For immobilized cultures, a stainless steel packing immersed in the culture broth is used as a support. In these conditions, the fungus exclusively grows on the packing, which provides a considerable advantage for astin C recovery and purification. C. asteris metabolism is different according to the culture conditions in terms of substrate consumption rate, cell growth, and astin C production. Immobilized-cell cultures yield a 30% increase of astin C production, associated with a 39% increase in biomass. The inoculum type as spores rather than hyphae, and a pre-inoculation washing procedure with sodium hydroxide, turns out to be beneficial both for astin C production and fungus development onto the support. Finally, the influence of culture parameters such as pH and medium composition on astin C production is evaluated. With optimized culture conditions, astin C yield is further improved reaching a five times higher final specific yield compared to the value reported with astin C extraction from A. tataricus (0.89 mg g−1 and 0.16 mg g−1 respectively).

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