Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 553020
Title Accumulation of intracellular trehalose and lactose in Lactobacillus plantarum WCFS1 during pulsed electric field treatment and subsequent freeze and spray drying
Author(s) Vaessen, Evelien M.J.; Besten, Heidy M.W. den; Esveld, Erik D.C.; Schutyser, Maarten A.I.
Source Food Science and Technology = Lebensmittel-Wissenschaft und Technologie 115 (2019). - ISSN 0023-6438
DOI https://doi.org/10.1016/j.lwt.2019.108478
Department(s) Food Process Engineering
Food Microbiology
Food Technology
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2019
Keyword(s) Disaccharide - Electroporation - Lactic acid bacteria - Probiotics - Survival
Abstract

Survival of bacteria during drying processes is required for products such as probiotics or starter cultures. In earlier studies high intracellular trehalose concentrations have been related to processing robustness during freezing and drying for several cell types. In this study we evaluated pulsed electric field (PEF) pre-treatment to increase intracellular trehalose in Lactobacillus plantarum WCFS1 and subsequent survival during freeze drying and storage in a trehalose matrix. Surprisingly, despite increased intracellular trehalose by PEF pre-treatment no enhanced survival was observed after drying and storage. Additional analysis of intracellular trehalose before and after freeze drying and spray drying revealed that during the drying process intracellular trehalose concentrations increased. Due to this increase the intracellular trehalose concentrations in the PEF pre-treated and control samples did not differ anymore. To explain what happens during the drying processes, results for trehalose were compared to similar experiments with lactose. A remarkable difference was observed between transfer of trehalose and lactose into cells during the freezing step before freeze drying, while both disaccharides gave similar cellular protection when used as freezing and drying matrix. Generated knowledge on transport of disaccharides into bacterial cells during freezing and drying can benefit processing of living bacterial formulations.

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