Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 553175
Title Seed maturation and post-harvest ripening negatively affect arabidopsis somatic embryogenesis
Author(s) Wu, Han; Chen, Baojian; Fiers, Martijn; Wróbel-Marek, Justyna; Kodde, Jan; Groot, Steven P.C.; Angenent, Gerco; Feng, Hui; Bentsink, Leónie; Boutilier, Kim
Source Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants 139 (2019)1. - ISSN 0167-6857 - p. 17 - 27.
DOI https://doi.org/10.1007/s11240-019-01658-8
Department(s) BIOS Plant Development Systems
Onderzoekschool EPS
EPS
Laboratory of Plant Physiology
Publication type Refereed Article in a scientific journal
Publication year 2019
Keyword(s) ABA - Arabidopsis - Auxin - Post-harvest ripening - ROS - Seed maturation - Somatic embryogenesis
Abstract

Plant development is highly malleable, as evidenced by the ability of cultured cells, tissues and organs to regenerate into whole plants in vitro. The ability of plants to regenerate in vitro is influenced by many different factors, including the donor plant growth conditions and the type of explant. Empirical trial and error manipulation of these and other culture parameters is the basis for improving plant regeneration protocols, but the mechanisms underlying the effects of these parameters on plant regeneration are unknown. Somatic embryogenesis (SE) is a type of in vitro plant regeneration where somatic/vegetative cells are induced to form embryos. Here we show that seed maturation is one of the parameters that affects the ability of germinating embryos to undergo auxin-induced somatic embryogenesis in Arabidopsis thaliana. Late maturation stage seeds harvested from yellow siliques have a higher capacity for somatic embryogenesis than seeds harvested later from brown siliques, a process that can be mimicked by post-harvest storage. Physiological and genetic analyses suggest that an oxidizing environment and ABA metabolism enhance the rate at which germinating embryos lose capacity to reactivate embryogenic growth. Our data suggest that there is a narrow window during late seed maturation in which embryogenic competence is reduced, and that this process also takes place, albeit more slowly, during seed storage. This knowledge provides a framework for identifying new plant totipotency factors and for directing efficient SE in systems that make use of mature seed explants.

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