Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 553804
Title Preprint - Congruence of location-specific transcriptional programs in intestinal organoids during long-term culture
Author(s) Hee, B. van der; Madsen, O.; Smidt, H.; Wells, J.M.
Source BioRxiv - 18 p.
Department(s) Host-Microbe Interactomics
Animal Breeding and Genomics
Publication type Working paper aimed at scientific audience
Publication year 2019
Keyword(s) Intestinal organoids - spheroids - RNAseq - IPEC-J2 - Transcriptome - Model similarity
Abstract The emergence of intestinal organoids, as a stem cell-based self-renewable model system, has led to many studies on intestinal development and cell-cell signaling. However, potential issues regarding the phenotypic stability and reproducibility of the methodology during culture still needs to be addressed for different organoids. Here we investigated the transcriptomes of intestinal organoids derived from the same pig as well as batch-to-batch variation of organoids derived from different pigs over long-term passage. The set of genes expressed in organoids closely resembled that of the tissue of origin, including location specific functions, for at least 17 passages. Minor differences in gene expression were observed between individual organoid cultures. In contrast, most tissue-specific genes were not expressed in the transformed jejunum cell line IPECJ2, which also showed gene expression consistent with cancer phenotypes. We conclude that intestinal organoids provide a robust and stable model for translational research with clear advantages over transformed cells.
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