Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 553809
Title 0046 - Organoids as models to study probiotics
Author(s) Hee, B. van der; Loonen, L.M.P.; Taverne, N.; Taverne-Thiele, J.J.; Smidt, H.; Wells, J.M.
Event IPC2019, Prague, 2019-06-17/2019-06-20
Department(s) Host-Microbe Interactomics
Publication type Abstract in scientific journal or proceedings
Publication year 2019
Keyword(s) permeability - intestinal models - organoids - stem-cells - tight-junctions
Abstract For decades, scientists have exploited cancer cell lines as models to study host-pathogen interactions and intestinal functions in vitro. Such monotypic cell models have led to important discoveries but have notable limitations. Immortalized cell lines display biological variations such as aneuploidy, chromosome rearrangements or mutations leading to poorly reproducible results, even for the same cell line.
Organoids are gaining considerable interest as alternative models of the intestine due to their close resemblance to structural, cellular and functional complexity found in vivo. However, the three-dimensional geometry of stem-cell derived intestinal organoids limits easy access to the apical epithelium for investigating the influence of probiotics, bioactive and toxic compounds on barrier function and permeability. Here we present a new robust method for generating confluent intestinal cell monolayers from single-cell suspensions of enzymatically-dissociated organoids. Confluent polarised monolayers containing tight-junctions were formed in three days and could be used in experiments for up to two weeks. Multilineage differentiation of the ileal stem cells was demonstrated by immunohistochemistry, and RT-qPCR of cell-specific transcripts. Furthermore, we showed that adult stem-cell derived ileal organoids maintain location-specific transcriptional programs during long-term in vitro culture.
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