Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 553878
Title Highly efficient mono-functionalization of knob-in-hole antibodies with strain-promoted click chemistry
Author(s) Bruins, Jorick J.; Wouw, Criss van de; Koen, Wagner; Lina, Bartels; Albada, Bauke; Delft, F.L. van
Source Journal of the American Chemical Society 4 (2019). - ISSN 0002-7863 - p. 11801 - 11807.
DOI https://doi.org/10.1021/acsomega.9b01727
Department(s) Organic Chemistry
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2019
Abstract Knob-in-hole antibodies can be utilized to introduce a single tag for chemo-enzymatic functionalization. By either introducing a single C-terminal sortase tag (sortase-tag expressed protein ligation) or tyrosine tag (G4Y), mono-functionalization of the monoclonal antibody trastuzumab was achieved rapidly and in high yields. This method was applied to selectively and efficiently introduce a single fluorescent tag, cytokine or single-chain variable fragment, as well as produce clean homo dimers of trastuzumab.

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