|Title||Highly efficient mono-functionalization of knob-in-hole antibodies with strain-promoted click chemistry|
|Author(s)||Bruins, Jorick J.; Wouw, Criss van de; Koen, Wagner; Lina, Bartels; Albada, Bauke; Delft, F.L. van|
|Source||Journal of the American Chemical Society 4 (2019). - ISSN 0002-7863 - p. 11801 - 11807.|
|Publication type||Refereed Article in a scientific journal|
|Abstract||Knob-in-hole antibodies can be utilized to introduce a single tag for chemo-enzymatic functionalization. By either introducing a single C-terminal sortase tag (sortase-tag expressed protein ligation) or tyrosine tag (G4Y), mono-functionalization of the monoclonal antibody trastuzumab was achieved rapidly and in high yields. This method was applied to selectively and efficiently introduce a single fluorescent tag, cytokine or single-chain variable fragment, as well as produce clean homo dimers of trastuzumab.