Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 554254
Title Effects of gut microbiota manipulation on ex vivo lipolysis in human abdominal subcutaneous adipocytes
Author(s) Jocken, Johan W.E.; Reijnders, Dorien; Canfora, Emanuel E.; Boekschoten, Mark V.; Plat, Joghum; Goossens, Gijs H.; Blaak, Ellen E.
Source Adipocyte 7 (2018)2. - ISSN 2162-3945 - p. 106 - 112.
DOI https://doi.org/10.1080/21623945.2018.1464366
Department(s) Sensory Science and Eating Behaviour
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Adipose Tissue - Fatty acid metabolism - Insulin resistance - Lipolysis - Microbiota - Obesity
Abstract

The intestinal microbiota may contribute to the development of obesity by affecting host lipid metabolism and insulin sensitivity. To investigate the effects of microbiota manipulation on ex vivo basal and β-adrenergically-stimulated lipolysis in human adipocytes, 36 obese men were randomized to amoxicillin (broad-spectrum antibiotic), vancomycin (narrow-spectrum antibiotic) or placebo treatment (7 d, 1500 mg/d). Before and after treatment, ex vivo adipose tissue lipolysis was assessed under basal conditions and during stimulation with the non-selective β-agonist isoprenaline using freshly isolated mature adipocytes. Gene (targeted microarray) and protein expression were analyzed to investigate underlying pathways. Antibiotics treatment did not significantly affect basal and maximal isoprenaline-mediated glycerol release from adipocytes. Adipose tissue β-adrenoceptor expression or post-receptor signalling was also not different between groups. In conclusion, 7 d oral antibiotics treatment has no effect on ex vivo lipolysis in mature adipocytes derived from adipose tissue of obese insulin resistant men.

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