Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 558258
Title Incomplete pole orientation of kinetochores in complex meiotic metaphase I configurations delays metaphase-anaphase transition in Secale
Author(s) Sybenga, J.
Source Genome 57 (2014)4. - ISSN 0831-2796 - p. 233 - 238.
DOI https://doi.org/10.1139/gen-2014-0067
Department(s) Laboratory of Genetics
Publication type Refereed Article in a scientific journal
Publication year 2014
Keyword(s) Anaphase checkpoint - Kinetochore activity - Meiosis - Multivalent orientation - Secale
Abstract

To prevent unbalanced chromosome segregation, meiotic metaphase I-anaphase I transition is carefully regulated by delaying anaphase until all kinetochores are well oriented (anaphase checkpoint) in mammals and insects. In plants this has not yet been established. In heterozygotes of two reciprocal translocations of Secale cereale, with one chromosome replaced by its two telocentric arms, anaphase delay was correlated with the orientation of the kinetochores of the complex of five chromosomes. The terminal kinetochores of the half chromosomes were readily elongated and pole oriented. Chains of five chromosomes with all five kinetochores orienting on alternate poles where the first to start anaphase. Kinetochores of two adjacent chromosomes when oriented on the same pole were partly shielded and less well pole directed. Anaphase was delayed. Cells with this configuration accumulated during anther development. Kinetochores in metacentric chromosomes lacking chiasmata in one arm (in trivalents and bivalents) were slightly better pole oriented and delayed anaphase less. Release of chromatid cohesion as triggered by kinetochore stretch is apparently delayed by inadequate exposition and pole orientation of the kinetochores. It is a mild form of an anaphase checkpoint, in normal material synchronizing bivalent segregation.

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