Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 558267
Title Not sequentially but simultaneously : Facile extraction of proteins and oleosomes from oilseeds
Author(s) Ntone, Eleni; Bitter, Johannes H.; Nikiforidis, Constantinos V.
Source Food Hydrocolloids 102 (2020). - ISSN 0268-005X
Department(s) Biobased Chemistry and Technology
Publication type Refereed Article in a scientific journal
Publication year 2020
Keyword(s) Emulsifiers - Oil bodies - Oil-in-water systems - Oleosomes - Plant proteins - Rapeseed proteins

Oilseeds represent a sustainable source of oils and proteins that can replace those of animal origin. However, the extraction of oil and proteins from oilseeds currently requires multiple steps and is plagued by undesired reactions occurring during the extraction, which limits valorization. In this paper, we describe a successful method for the simple simultaneous extraction of proteins and oil (as intact oleosomes). Non-defatted dehulled rapeseeds served as oilseed model. First, an aqueous extraction step at pH 9.0 was performed resulting in a protein-oleosome extract, with extraction yields of 78.8 ±0.2 wt% and 82.8 ±0.4 wt% of proteins and oleosomes respectively. Further separation resulted in a protein-rich and an oleosome-rich mixture. The oleosomes were recovered as high oil volume oil-in-water emulsion, while simple filtration of the protein-rich mixture led to a highly soluble (81.4 ±1.9 wt%) protein concentrate. Following this extraction method, complexation between proteins and phenolic compounds was prevented, a clear advantage over the existing methods. These findings emphasize the importance of designing new processes for the extraction of oilseed proteins and oleosomes that could initiate their use in food systems.

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