Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 558279
Title Impact of water degumming and enzymatic degumming on gum mesostructure formation in crude soybean oil
Author(s) Nikolaeva, Tatiana; Rietkerk, Tim; Sein, Arjen; Dalgliesh, Robert; Bouwman, Wim G.; Velichko, Evgenii; Tian, Bei; As, Henk Van; Duynhoven, John van
Source Food Chemistry 311 (2020). - ISSN 0308-8146
DOI https://doi.org/10.1016/j.foodchem.2019.126017
Department(s) Biophysics
EPS
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2020
Keyword(s) Enzymatic degumming - Lamellar liquid-crystalline structure - Phospholipid - Water degumming
Abstract

Phospholipid gum mesostructures formed in crude soybean oil after water degumming (WD) and enzymatic degumming (ED) were studied at a range of phospholipid and water concentrations. For ED, phospholipase C (PLC), phospholipase A2 (PLA2) and a mixture of phospholipases Purifine 3G (3G) were used. Both WD and ED resulted in lamellar liquid-crystalline phases, however, of different topology. The dependence of the bilayer spacings (as observed by SANS and SAXS) on the ratio between amount of water and amphiphilic lipids differed for WD and PLA2 ED vs PLC and 3G ED. This difference was also observed for dynamics at molecular scale as observed by time-domain (TD) NMR and attributed to partial incorporation of diglycerides and free fatty acids into gum bilayers after PLC and 3G ED. Feasibility of using TD-NMR relaxometry for quantification of the gum phase and estimation of degumming efficiency was demonstrated.

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