Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 558546
Title Effect of growth conditions on the efficiency of cell disruption of Neochloris oleoabundans
Author(s) Safi, C.; Olivieri, G.; Engelen-Smit, N.; Spekking, W.; Veloo, R.; Broek, L.A.M. van den; Sijtsma, L.
Source Bioresource Technology 300 (2020). - ISSN 0960-8524
Department(s) BBP Biorefinery & Sustainable Value Chains
Bioprocess Engineering
BBP Bioconversion
Publication type Refereed Article in a scientific journal
Publication year 2020
Keyword(s) Cell disintegration - Enzymes - High-pressure homogenization - Nitrogen deplete - Nitrogen replete

The impact of four different growth conditions on the cell disruption efficiency of Neochloris oleoabundans was investigated. A mechanical and biological cell disruption methods were evaluated separately and combined. It has been established that microalgae grown in marine water under nitrogen deprivation were the most resistant against cell disruption methods and released the lowest amount of proteins. The release of lipids, however, followed the “hindered molecule diffusion phenomenon” because it did not follow the same release pattern as proteins. The enzymatic treatment was efficient enough to release the majority of the proteins without combining it with high-pressure homogenization. Regarding energy input, Neochloris oleoabundans grown in marine water under nitrogen deprivation required the highest energy input to release proteins (Ep = 13.76−1) and to break the cells by high-pressure homogenization (Ex – HPH = 1.14−1) or by the combination of enzymes and High-pressure homogenization (Ex – ENZ = 2.79−1).

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