Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 558576
Title Chemokine CXCb1 stimulates formation of NETs in trunk kidney neutrophils of common carp
Author(s) Pijanowski, Lukasz; Verburg van Kemenade, Lidy; Chadzinska, Magdalena
Source Developmental and Comparative Immunology 103 (2020). - ISSN 0145-305X
DOI https://doi.org/10.1016/j.dci.2019.103521
Department(s) WIAS
Cell Biology and Immunology
Publication type Refereed Article in a scientific journal
Publication year 2020
Keyword(s) CXC chemokines - Histone H3 - NADPH oxidase - Neutrophil elastase - Neutrophil extracellular traps
Abstract

Both in mammals and in fish, CXC chemokines activate leukocytes and regulate their migration both under normal physiological and inflammatory conditions. Moreover, in mammalian neutrophils CXC chemokines also stimulate the formation of neutrophil extracellular traps (NETs). Here, we investigated the effects of recombinant carp CXCL8s and CXCb1 on NET formation in neutrophils from the head (HK) and trunk (TK) kidney of carp. We found that neither recombinant CXCL8s nor CXCb1 stimulated DNA release in HK-derived neutrophils, while in TK-derived cells rcCXCb1 stimulated the release of NETs, composed of extracellular DNA co-localized with citrulline H3 histone and neutrophil elastase. Furthermore, CXCb1-induced NET release required NADPH oxidase activity, while it did not change upon treatment with CXCR inhibitors. In conclusion, we demonstrated, for the first time in fish, that CXCb1 chemokine induces formation of NETs in TK-derived neutrophils and this process is ROS-dependent. The difference between HK and TK-derived neutrophils is probably related to differences in the maturation state of these cells.

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