Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 558586
Title Direct Visualization of Native CRISPR Target Search in Live Bacteria Reveals Cascade DNA Surveillance Mechanism
Author(s) Vink, Jochem N.A.; Martens, Koen J.A.; Vlot, Marnix; McKenzie, Rebecca E.; Almendros, Cristóbal; Estrada Bonilla, Boris; Brocken, Daan J.W.; Hohlbein, Johannes; Brouns, Stan J.J.
Source Molecular Cell 77 (2020)1. - ISSN 1097-2765 - p. 39 - 50.e10.
DOI https://doi.org/10.1016/j.molcel.2019.10.021
Department(s) Biophysics
Microbiology
VLAG
EPS
Publication type Refereed Article in a scientific journal
Publication year 2020
Keyword(s) arms race - Cascade - CRISPR-Cas - PALM - PAM - photo-activation localization microscopy - single molecule - single-particle tracking - target search
Abstract

Vink et al. tracked single CRISPR RNA-surveillance complexes (Cascade) in the native host cell and determined the influence of Cascade copy numbers, PAM scanning speed, and the presence of CRISPR arrays and transcription on their ability to find and clear invading mobile genetic elements from the cell.

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