Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 558602
Title Preliminary Evaluation of Aqueous Protein Extraction from Black Soldier Fly Larvae (Hermetia illucens L.)
Author(s) Miron, Lucian T.; Postma, Richard P.; Bosch, Guido; Eppink, Michel H.M.
Source Industrial Biotechnology 15 (2019)6. - ISSN 1550-9087 - p. 365 - 369.
DOI https://doi.org/10.1089/ind.2019.0017
Department(s) Bioprocess Engineering
WIAS
Animal Nutrition
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2019
Keyword(s) black soldier fly larvae - insects - protein extraction - SDS-PAGE - soluble protein
Abstract

This study aimed to explore the efficacy of mild water-soluble protein extraction procedures on black soldier fly larvae (BSFL), a potential alternative and sustainable protein source for food. Different conditions were tested for extraction of water-soluble proteins including phosphate buffered saline (PBS) and Tris-HCl buffer in a pH range from 7.0 to 9.0. The total protein content and the water-soluble protein content were measured by Lowry BioRad DC™ protein assay. The molecular weight distribution of water-soluble proteins was characterized using SDS-PAGE. The black soldier fly larvae contained 42.3% protein on a dry matter basis, which is comparable to other studies. After extraction, the water-soluble protein fraction ranged from 5.5 to 17.2%, dependent on the buffer conditions, and total protein content and discoloration of the supernatant was observed, which could be an indication of oxidation. Moreover, the SDS-PAGE method allowed the determination of molecular weight distribution of the protein fraction showing no protein degradation with discrete bands and the most intensive band <75 kDa was observed. The extraction of water-soluble BSFL proteins was mild, easy, fast and in agreement with other studies. Further research should focus on the characterization and techno-functional properties of the aqueous protein extracts as food ingredient alternative.

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