Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 558982
Title Structure-function analysis of the coxsackievirus protein 3A : Identification of residues important for dimerization, viral RNA replication, and transport inhibition
Author(s) Wessels, Els; Notebaart, Richard A.; Duijsings, Daniël; Lanke, Kjerstin; Vergeer, Bart; Melchers, Willem J.G.; Kuppeveld, Frank J.M. Van
Source Journal of Biological Chemistry 281 (2006)38. - ISSN 0021-9258 - p. 28232 - 28243.
Publication type Refereed Article in a scientific journal
Publication year 2006

The coxsackievirus B3 3A protein forms homodimers and plays important roles in both viral RNA (vRNA) replication and the viral inhibition of intracellular protein transport. The molecular determinants that are required for each of these functions are yet poorly understood. Based on the NMR structure of the closely related poliovirus 3A protein, a molecular model of the coxsackievirus B3 3A protein was constructed. Using this structural model, specific mutants were designed to study the structure-function relationship of 3A. The mutants were tested for their capacity to dimerize, support vRNA replication, and block protein transport. A hydrophobic interaction between the monomers and an intermolecular salt bridge were identified as major determinants required for dimerization. We show that dimerization is important for both efficient vRNA replication and inhibition of protein transport. In addition, determinants were identified that were not required for dimerization but that were essential for either one of the biological functions of 3A. The combination of the in silico and in vivo results obtained in this study provides important insights in both the structural and functional aspects of 3A.

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