Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 559331
Title Molecular binding between anthocyanins and pectic polysaccharides – Unveiling the role of pectic polysaccharides structure
Author(s) Fernandes, Ana; Oliveira, Joana; Fonseca, Fátima; Ferreira-da-Silva, Frederico; Mateus, Nuno; Vincken, Jean Paul; Freitas, Victor de
Source Food Hydrocolloids 102 (2020). - ISSN 0268-005X
DOI https://doi.org/10.1016/j.foodhyd.2019.105625
Department(s) VLAG
Food Chemistry
Publication type Refereed Article in a scientific journal
Publication year 2020
Keyword(s) Anthocyanins - Cyanidin-3-O-Glucoside - ITC - NMR - Pectic polysaccharides - UV–Visible
Abstract

The interaction between cyanidin-3-O-glucoside and four citrus pectic fractions was investigated using a combined molecular approach, including isothermal titration calorimetry (ITC), magnetic nuclear resonance (NMR) and UV–Visible spectrophotometry. These pectic fractions differed on their type and degree of esterification (amidated (AHG 30%), low (HG 30%) and high methyl esterified fractions (HG 70%)) and on the pattern of esterification (blockwise (HG-B 70%) vrs random (HG-R 70%)). The binding constant (Ka) and the associated thermodynamic parameters determined showed weak noncovalent interactions, particularly due to electrostatic interactions, hydrogen bonding and hydrophobic effect. The degree and the type of esterification of each pectic fractions had a significant effect on the binding constants determined by ITC and NMR experiments. The binding constants ranged from 102 to 104 M−1, with the highest Ka value observed for the interaction between cy3glc and low methylesterified fraction, followed by the amidated fraction. Pectic fractions with a higher degree of methyl esterification, resulted on lower binding affinities, with these interactions being mostly driven by hydrophobic effect compared to enthalpy. The different binding affinities could be correlated with anthocyanins colour impact, as a higher red colour intensity could be observed for a cy3glc model solution fortified with a low methylated fraction. However, this colour improvement was not observed for amidated fraction, presumably due to charge repulsive forces, lowering the stability of the flavylium cation.

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