Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 559408
Title High-level integration of murine intestinal transcriptomics data highlights the importance of the complement system in mucosal homeostasis
Author(s) Benis, Nirupama; Wells, Jerry M.; Smits, Mari A.; Kar, Soumya Kanti; Hee, Bart van der; Martins dos Santos, Vitor A.P.; Suarez-Diez, Maria; Schokker, Dirkjan
Source BMC Genomics 20 (2019)1. - ISSN 1471-2164
DOI https://doi.org/10.1186/s12864-019-6390-x
Department(s) Systems and Synthetic Biology
Host-Microbe Interactomics
WIAS
VLAG
Infection Biology
Animal Nutrition
Animal Breeding & Genomics
Publication type Refereed Article in a scientific journal
Publication year 2019
Keyword(s) Complement pathway - Data integration - Homeostasis - Intestine - Pathway analysis - Transcriptomics
Abstract

Background: The mammalian intestine is a complex biological system that exhibits functional plasticity in its response to diverse stimuli to maintain homeostasis. To improve our understanding of this plasticity, we performed a high-level data integration of 14 whole-genome transcriptomics datasets from samples of intestinal mouse mucosa. We used the tool Centrality based Pathway Analysis (CePa), along with information from the Reactome database. Results: The results show an integrated response of the mouse intestinal mucosa to challenges with agents introduced orally that were expected to perturb homeostasis. We observed that a common set of pathways respond to different stimuli, of which the most reactive was the Regulation of Complement Cascade pathway. Altered expression of the Regulation of Complement Cascade pathway was verified in mouse organoids challenged with different stimuli in vitro. Conclusions: Results of the integrated transcriptomics analysis and data driven experiment suggest an important role of epithelial production of complement and host complement defence factors in the maintenance of homeostasis.

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