Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 559510
Title Trypanosome-host interaction revealed through the zebrafish looking glass
Author(s) Jacobs, S.H.; Doro, E.; Hammond, Ffion; Brugman, S.; Nguyen-Chi, Mai; Wiegertjes, G.; Forlenza, M.
Source Fish and Shellfish Immunology 91 (2019). - ISSN 1050-4648 - p. 443 - 443.
Event International Fish & Shellfish Immunology Congress 2019, Gran Canaria, 2019-06-16/2019-06-20
Department(s) Cell Biology and Immunology
Publication type Abstract in scientific journal or proceedings
Publication year 2019
Abstract Trypanosoma carassii is an extracellular blood parasite of cyprinid fish phylogenetically closely related to Trypanosoma brucei, the causative agent of the sleeping sickness disease in humans and livestock. Motility is crucial for trypanosome pathogenicity, but real-time visualization of parasite movement in vivo, in the natural host environment, has not been reported thus far. In this study, we report the establishment of T. carassii infection in zebrafish (Danio rerio), which allowed us, for the first time in a vertebrate host, to characterize in details the movement of trypanosomes in vivo. By combining the transparency of zebrafish larvae with the availability of several transgenic lines marking macrophages, neutrophils, cytokine-expressing leukocytes and endothelial cells, we were able to study in real-time: 1) parasite movement in vivo; 2) the kinetics of innate immune responses; and 3) parasite interaction with host (immune) cells. Our results indicate that during T. carassii infection of young zebrafish a differential macrophages and neutrophils response is observed. Macrophages responded more prominently than neutrophils by proliferating, and were massively recruited to blood vessels. Macrophages also exhibited heterogeneous morphologies and a strong pro-inflammatory profile. In fact, they were strongly positive for Tnfα and Il-1β and had a morphology characteristic of foamy macrophages. Large foamy macrophages accumulated in the portal vein of highly infected individuals, and were strongly positive for lipid staining, which revealed the abundance of lipid bodies in their cytoplasm. Finally, with respect to parasite movement and interaction with the host, using high-speed videography, we were able to capture novel mechanisms of parasite-host cell interaction, and to follow the onset of anemia, vasodilation and extravasation typical of trypanosome infections. Altogether, this is the first report of an in vivo trypanosome infection model in a natural vertebrate host describing both, the pathogen behavior and the host response. Considering that trypanosomes can infect all vertebrates, including humans, livestock and fish, our infection model is a relevant complementary tool to gain more insights in the underlying mechanisms of trypanosome infections.
There are no comments yet. You can post the first one!
Post a comment
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.