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Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 559512
Title Generation of tools for characterization of adaptive immune response in carp
Author(s) Awatif, Eltijani; Embregts, C.W.E.; Brugman, S.; Wiegertjes, G.; Forlenza, M.
Source Fish and Shellfish Immunology 91 (2019). - ISSN 1050-4648 - p. 420 - 420.
Event International Fish & Shellfish Immunology Congress 2019, Gran Canaria, 2019-06-16/2019-06-20
Department(s) Cell Biology and Immunology
Aquaculture and Fisheries
Publication type Abstract in scientific journal or proceedings
Publication year 2019
Abstract Recently our group reported the development of an intramuscular DNA vaccine against Spring Viremia of Carp Virus (SVCV). This vaccine conferred up to 100% protection to carp even at a single dose of 0.1 μg DNA/g of fish. A further characterization of the adaptive immune response revealed the presence of serum neutralizing antibodies and of memory T cells in vaccine-protected animals. Nevertheless, with the current tools, it was not possible to determine which specific type of immunoglobulin (IgM, IgD, IgT1, IgT2 or all) contributed to the serum neutralizing titres, nor which specific subtype of T cells (CD4+, CD8α+ or both) contributed to the memory response.
In this study, we report the preliminary characterization of tools against B and t cells of carp. For example, we have developed polyclonal antibodies recognizing IgT1+ positive B cells and are further characterizing a monoclonal antibody recognizing putative mucosal T cells. These tools, combined with those already available in our group against IgM+ B cells, CD4-1+ and CD8a+ T cells, provide a panel of markers that will allow the characterization of B and T cell adaptive immune response in carp in general and to vaccination in particular.
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