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Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 559667
Title Piper anisum as a promising new source of bioactive metabolites
Author(s) Batista, Danilo; Campos, Patrícia; Silva, Valdenizia R.; S. Santos, Luciano de; Bezerra, Daniel P.; Soares, Milena B.P.; Colepicolo, Pio; Zambotti-Villela, Leonardo; Pinto, Ernani; Araújo, Floricea M.; Martins, Dirceu; Fernandez, Luzimar G.; Ligterink, Wilco; Canuto, Gisele A.B.; Cerqueira, Martins Dias de; Ribeiro, Paulo R.
Source Chemical Papers 74 (2019). - ISSN 0366-6352 - p. 1505 - 1515.
Department(s) EPS
Laboratory of Plant Physiology
Publication type Refereed Article in a scientific journal
Publication year 2019
Keyword(s) Antimicrobial compounds - Antioxidant capacity - Antitumor activity - Bioactive metabolites - Ethnopharmacology - Metabolomics

Piper species are commonly used by indigenous communities to treat several gastrointestinal diseases. In China, they are also used as an active ingredient in formulae to treat cancer. The objective of the study was to perform a large-scale metabolite profiling analysis to identify bioactive compounds in Piper anisum. Antioxidant capacity was assessed by the DPPH assay and total phenolics were assessed by Folin–Ciocalteu’s method. Antimicrobial activity was assessed against several Gram-positive and Gram-negative bacteria, whereas cytotoxicity was assessed against tumor cell lines MCF-7, HCT116, HepG2 and HL-60, and non-tumor cell line MRC-5. The multiplatform metabolite profiling approach encompassed NMR, GC–MS and LC–MS analyses. P. anisum root extract showed the greatest antioxidant capacity and total phenolic content, followed by the stem and leaf extracts. P. anisum extracts showed a highly selective antimicrobial profile, being specifically active against C. albicans (MIC of 500 μg mL−1). Additionally, the root extract (50 μg mL−1) showed the highest cell inhibition percentages against tumor cell lines MCF-7 (59.5%), HCT116 (49.2%), and HepG2 (61.0%). Forty-eight metabolites were annotated by GC–MS and 27 by LC–MS. These included alkaloids, carbohydrates, fatty acids, hydrocarbons, organic acids, phenolic compounds, and terpenes. Taken together, these results showed that P. anisum root extract is a promising source of bioactive compounds.

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