Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 559695
Title Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants
Author(s) Makarova, Kira S.; Wolf, Yuri I.; Iranzo, Jaime; Shmakov, Sergey A.; Alkhnbashi, Omer S.; Brouns, Stan J.J.; Charpentier, Emmanuelle; Cheng, David; Haft, Daniel H.; Horvath, Philippe; Moineau, Sylvain; Mojica, Francisco J.M.; Scott, David; Shah, Shiraz A.; Siksnys, Virginijus; Terns, Michael P.; Venclovas, Česlovas; White, Malcolm F.; Yakunin, Alexander F.; Yan, Winston; Zhang, Feng; Garrett, Roger A.; Backofen, Rolf; Oost, John van der; Barrangou, Rodolphe; Koonin, Eugene V.
Source Nature Reviews Microbiology 18 (2020). - ISSN 1740-1526 - p. 67 - 83.
DOI https://doi.org/10.1038/s41579-019-0299-x
Department(s) VLAG
Microbiology
Publication type Refereed Article in a scientific journal
Publication year 2020
Abstract

The number and diversity of known CRISPR–Cas systems have substantially increased in recent years. Here, we provide an updated evolutionary classification of CRISPR–Cas systems and cas genes, with an emphasis on the major developments that have occurred since the publication of the latest classification, in 2015. The new classification includes 2 classes, 6 types and 33 subtypes, compared with 5 types and 16 subtypes in 2015. A key development is the ongoing discovery of multiple, novel class 2 CRISPR–Cas systems, which now include 3 types and 17 subtypes. A second major novelty is the discovery of numerous derived CRISPR–Cas variants, often associated with mobile genetic elements that lack the nucleases required for interference. Some of these variants are involved in RNA-guided transposition, whereas others are predicted to perform functions distinct from adaptive immunity that remain to be characterized experimentally. The third highlight is the discovery of numerous families of ancillary CRISPR-linked genes, often implicated in signal transduction. Together, these findings substantially clarify the functional diversity and evolutionary history of CRISPR–Cas.

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