Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 559900
Title Reciprocal cybrids reveal how organellar genomes affect plant phenotypes
Author(s) Flood, Pádraic J.; Theeuwen, Tom P.J.M.; Schneeberger, Korbinian; Keizer, Paul; Kruijer, Willem; Severing, Edouard; Kouklas, Evangelos; Hageman, Jos A.; Wijfjes, Raúl; Calvo-Baltanas, Vanesa; Becker, Frank F.M.; Schnabel, Sabine K.; Willems, Leo A.J.; Ligterink, Wilco; Arkel, Jeroen Van; Mumm, Roland; Gualberto, José M.; Savage, Linda; Kramer, David M.; Keurentjes, Joost J.B.; Eeuwijk, Fred Van; Koornneef, Maarten; Harbinson, Jeremy; Aarts, Mark G.M.; Wijnker, Erik
Source Nature Plants 6 (2020)1. - ISSN 2055-026X - p. 13 - 21.
DOI https://doi.org/10.1038/s41477-019-0575-9
Department(s) Horticulture & Product Physiology
Laboratory of Genetics
Biometris
Mathematical and Statistical Methods - Biometris
Bioinformatics
PE&RC
Laboratory of Plant Physiology
EPS
BIOS Applied Metabolic Systems
Publication type Refereed Article in a scientific journal
Publication year 2020
Abstract Assessment of the impact of variation in chloroplast and mitochondrial DNA (collectively termed the plasmotype) on plant phenotypes is challenging due to the difficulty in separating their effect from nuclear-derived variation (the nucleotype). Haploid-inducer lines can be used as efficient plasmotype donors to generate new plasmotype–nucleotype combinations (cybrids)1. We generated a panel comprising all possible cybrids of seven Arabidopsis thaliana accessions and extensively phenotyped these lines for 1,859 phenotypes under both stable and fluctuating conditions. We show that natural variation in the plasmotype results in both additive and epistatic effects across all phenotypic categories. Plasmotypes that induce more additive phenotypic changes also cause more epistatic effects, suggesting a possible common basis for both additive and epistatic effects. On average, epistatic interactions explained twice as much of the variance in phenotypes as additive plasmotype effects. The impact of plasmotypic variation was also more pronounced under fluctuating and stressful environmental conditions. Thus, the phenotypic impact of variation in plasmotypes is the outcome of multi-level nucleotype–plasmotype–environment interactions and, as such, the plasmotype is likely to serve as a reservoir of variation that is predominantly exposed under certain conditions. The production of cybrids using haploid inducers is a rapid and precise method for assessment of the phenotypic effects of natural variation in organellar genomes. It will facilitate efficient screening of unique nucleotype–plasmotype combinations to both improve our understanding of natural variation in these combinations and identify favourable combinations to enhance plant performance.
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