Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 560423
Title Meiotic recombination profiling of interspecific hybrid F1 tomato pollen by linked read sequencing
Author(s) Fuentes, Roven; Hesselink, T.; Nieuwenhuis, Ronald; Bakker, L.V.; Schijlen, E.G.W.M.; Dooijeweert, W. van; Diaz Trivino, S.; Haan, Jorn R. de; Sanchez Perez, G.F.; Zhang, Xinyue; Fransz, Paul; Jong, J.H.S.G.M. de; Dijk, A.D.J. van; Ridder, D. de; Peters, S.A.
Source The Plant Journal (2019). - ISSN 0960-7412
DOI https://doi.org/10.1111/tpj.14640
Department(s) Bioinformatics
BIOS Applied Metabolic Systems
BIOS Applied Bioinformatics
WOT CGN
Laboratory of Genetics
EPS
Mathematical and Statistical Methods - Biometris
Publication type Refereed Article in a scientific journal
Publication year 2019
Abstract Genome wide screening of pooled pollen samples from a single interspecific F1 hybrid obtained from a cross between tomato, Solanum lycopersicum and its wild relative, Solanum pimpinellifolium using linked read sequencing of the haploid nuclei, allowed profiling of the crossover (CO) and gene conversion (GC) landscape. We observed a striking overlap between cold regions of CO in the male gametes and our previously established F6 recombinant inbred lines (RILs) population. COs were overrepresented in non‐coding regions in the gene promoter and 5′UTR regions of genes. Poly‐A/T and AT rich motifs were found enriched in 1 kb promoter regions flanking the CO sites. Non‐crossover associated allelic and ectopic GCs were detected in most chromosomes, confirming that besides CO, GC represents also a source for genetic diversity and genome plasticity in tomato. Furthermore, we identified processed break junctions pointing at the involvement of both homology directed and non‐homology directed repair pathways, suggesting a recombination machinery in tomato that is more complex than currently anticipated.
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