Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 560652
Title Different carbon sources result in differential activation of sigma B and stress resistance in Listeria monocytogenes
Author(s) Crespo Tapia, Natalia; Dorey, Amber L.; Gahan, Cormac G.M.; Besten, Heidy M.W. den; O'Byrne, Conor P.; Abee, Tjakko
Source International Journal of Food Microbiology 320 (2020). - ISSN 0168-1605
DOI https://doi.org/10.1016/j.ijfoodmicro.2019.108504
Department(s) Food Microbiology
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2020
Keyword(s) Acid stress - Adhesion assay - Biofilm - C2Bbe1 cell line - Carbon source - Glucose - Glycerol - Heat stress - Invasion assay - Lactose - Listeria monocytogenes - SigB - Sigma B - Stress - Stress response - Virulence
Abstract

Listeria monocytogenes is an important food-borne pathogen that is ubiquitous in the environment. It is able to utilize a variety of carbon sources, to produce biofilms on food-processing surfaces and to survive food preservation–associated stresses. In this study, we investigated the effect of three common carbon sources, namely glucose, glycerol and lactose, on growth and activation of the general stress response Sigma factor, SigB, and corresponding phenotypes including stress resistance. A fluorescent reporter coupled to the promoter of lmo2230, a highly SigB-dependent gene, was used to determine SigB activation via quantitative fluorescence spectroscopy. This approach, combined with Western blotting and fluorescence microscopy, showed the highest SigB activation in lactose grown cells and lowest in glucose grown cells. In line with this observation, lactose grown cells showed the highest resistance to lethal heat and acid stress, the highest biofilm formation, and had the highest adhesion/invasion capacity in Caco-2-derived C2Bbe1 cell lines. Our data suggest that lactose utilisation triggers a strong SigB dependent stress response and this may have implications for the resistance of L. monocytogenes along the food chain.

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