Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 560672
Title Selective fractionation of free glucose and starch from microalgae using aqueous two-phase systems
Author(s) Suarez Ruiz, Catalina A.; Zarate Baca, Santiago; Broek, Lambertus A.M. van den; Berg, Corjan van den; Wijffels, Rene H.; Eppink, Michel H.M.
Source Algal Research 46 (2020). - ISSN 2211-9264
DOI https://doi.org/10.1016/j.algal.2020.101801
Department(s) Bioprocess Engineering
BBP Bioconversion
BBP Biorefinery & Sustainable Value Chains
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2020
Keyword(s) Aqueous two-phase systems - Carbohydrates - Extraction - Glucose - Ionic liquids - Microalgae
Abstract

Microalgae are a promising source of lipids, pigments, proteins and carbohydrates, which are valuable compounds for many industries. However, optimal fractionation and valorization of all produced compounds is necessary to improve the economic viability of microalgae production. This paper aims to understand the fractionation of microalgae carbohydrates (free glucose and starch) in aqueous two-phase systems. Three aqueous two-phase systems were investigated to efficiently and mildly separate carbohydrates from disrupted Neochloris oleoabundans. This strain contains 16 w/w% of proteins, 48 w/w% total fatty acids and 27 w/w% carbohydrates when cultivated under saline water and nitrogen depletion conditions. The protein content decreases and the amount of fatty acids and carbohydrates increases notably under stress conditions and glucose becomes the main carbohydrate in this microalgae. Glucose is present in the disrupted microalgae as part of polymeric carbohydrates (starch) or in monomeric form (free glucose). With the aqueous two-phase system Polyethylene Glycol 400 - Cholinium dihydrogen phosphate (PEG400-ChDHp) microalgal free glucose is fractionated up to a recovery of 99% to the most hydrated bottom phase in a single step. Simultaneously, a recovery of 70% is reached for microalgal starch in the interface after two additional liquid-liquid extractions with PEG400-ChDHp. The final fractions obtained were free of pigments.

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