Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 560843
Title Miniaturised interaction proteomics on a microfluidic platform with ultra-low input requirements
Author(s) Furlan, Cristina; Dirks, René A.M.; Thomas, Peter C.; Jones, Robert C.; Wang, Jing; Lynch, Mark; Marks, Hendrik; Vermeulen, Michiel
Source Nature Communications 10 (2019)1. - ISSN 2041-1723
DOI https://doi.org/10.1038/s41467-019-09533-y
Publication type Refereed Article in a scientific journal
Publication year 2019
Abstract

Essentially all cellular processes are orchestrated by protein-protein interactions (PPIs). In recent years, affinity purification coupled to mass spectrometry (AP-MS) has been the preferred method to identify cellular PPIs. Here we present a microfluidic-based AP-MS workflow, called on-chip AP-MS, to identify PPIs using minute amounts of input material. By using this automated platform we purify the human Cohesin, CCC and Mediator complexes from as little as 4 micrograms of input lysate, representing a 50─100-fold downscaling compared to regular microcentrifuge tube-based protocols. We show that our platform can be used to affinity purify tagged baits as well as native cellular proteins and their interaction partners. As such, our method holds great promise for future biological and clinical AP-MS applications in which sample amounts are limited.

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