|Title||Identification of Casuarina-Frankia strains by use of polymerase chain reaction (PCR) with arbitrary primers|
|Author(s)||Sellstedt, Anita; Wullings, Bart; Nyström, Ulrika; Gustafsson, Petter|
|Source||FEMS Microbiology Letters 93 (1992)1. - ISSN 0378-1097 - p. 1 - 5.|
|Publication type||Refereed Article in a scientific journal|
|Keyword(s)||Casuarinaceae - Frankia - Identification - Polymerase chain reaction - Polymorphism|
Free-living N2-fixing Frankia strains isolated from Casuarina sp. were investigated for genomic polymorphism. We used six 10-mer oligonucleotides as single arbitrary primers (AP) for the polymerase chain reaction (PCR) in order to amplify random DNA fragments in the genome of free-living Frankia strains. Agarose-gels of the amplified genomic DNA revealed that two of the six arbitrary primers showed polymorphism in the eight different Frankia genomes. Analysis of the AP-PCR products showed 9 polymorphic bands ranging from 4.1-0.60 kb. We conclude that single arbitrary primers can be used to amplify genomic DNA, and that polymorphism can be detected between the amplification products of the different Frankia genomes.