Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 560869
Title Identification of Casuarina-Frankia strains by use of polymerase chain reaction (PCR) with arbitrary primers
Author(s) Sellstedt, Anita; Wullings, Bart; Nyström, Ulrika; Gustafsson, Petter
Source FEMS Microbiology Letters 93 (1992)1. - ISSN 0378-1097 - p. 1 - 5.
Publication type Refereed Article in a scientific journal
Publication year 1992
Keyword(s) Casuarinaceae - Frankia - Identification - Polymerase chain reaction - Polymorphism

Free-living N2-fixing Frankia strains isolated from Casuarina sp. were investigated for genomic polymorphism. We used six 10-mer oligonucleotides as single arbitrary primers (AP) for the polymerase chain reaction (PCR) in order to amplify random DNA fragments in the genome of free-living Frankia strains. Agarose-gels of the amplified genomic DNA revealed that two of the six arbitrary primers showed polymorphism in the eight different Frankia genomes. Analysis of the AP-PCR products showed 9 polymorphic bands ranging from 4.1-0.60 kb. We conclude that single arbitrary primers can be used to amplify genomic DNA, and that polymorphism can be detected between the amplification products of the different Frankia genomes.

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