Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 560946
Title Cell type-specific transcriptomics in the plant embryo using an adapted INTACT protocol
Author(s) Palovaara, Joakim; Weijers, Dolf
Source In: Plant Embryogenesis / Bayer, M., New York : Humana Press Inc. (Methods in Molecular Biology ) - ISBN 9781071603413 - p. 141 - 150.
DOI https://doi.org/10.1007/978-1-0716-0342-0_11
Department(s) EPS
Biochemistry
Publication type Peer reviewed book chapter
Publication year 2020
Keyword(s) Cell type-specific - Embryo - Isolation - Nucleus - Transcriptome
Abstract

Cells differentiate from undifferentiated precursors in order to establish the tissues of vascular plants. The different cell types and stem cells are first specified in the early embryo. How cell type specification is instructed by transcriptional control on a genome-wide level is poorly understood. A major hurdle has been the technical challenge associated with obtaining cellular transcriptomes in this inaccessible tissue. Recently, we adapted a two-component genetic labeling system called INTACT to isolate nuclei and generate a microarray-based expression atlas of the cell types in the early Arabidopsis thaliana embryo. Here we present a step-by-step description of the adapted INTACT protocol, as well as the approach to generate transcriptomic profiles. This protocol has been adapted to account for using seeds with embryos of various developmental stages as a starting material, and the relatively few cell type-specific nuclei that can be isolated from embryos.

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