Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 560947
Title Analyzing subcellular reorganization during early Arabidopsis embryogenesis using fluorescent markers
Author(s) Liao, Che Yang; Weijers, Dolf
Source In: Plant Embryogenesis / Bayer, M., New York : Humana Press Inc. (Methods in Molecular Biology ) - ISBN 9781071603413 - p. 49 - 61.
DOI https://doi.org/10.1007/978-1-0716-0342-0_5
Department(s) Biochemistry
EPS
Publication type Peer reviewed book chapter
Publication year 2020
Keyword(s) Arabidopsis thaliana - Confocal microscopy - Embryogenesis - Fluorescent protein - Subcellular structure
Abstract

Virtually all growth, developmental, physiological, and defense responses in plants are accompanied by reorganization of subcellular structures to enable altered cellular growth, differentiation or function. Visualizing cellular reorganization is therefore critical to understand plant biology at the cellular scale. Fluorescently labeled markers for organelles, or for cellular components are widely used in combination with confocal microscopy to visualize cellular reorganization. Early during plant embryogenesis, the precursors for all major tissues of the seedling are established, and in Arabidopsis, this entails a set of nearly invariant switches in cell division orientation and directional cell expansion. Given that these cellular reorganization events are genetically regulated and coupled to formative events in plant development, they offer a good model to understand the genetic control of cellular reorganization in plant development. Until recently, it has been challenging to visualize subcellular structures in the early Arabidopsis embryo for two reasons: embryos are deeply embedded in seed coat and fruit, and in addition, no dedicated fluorescent markers, expressed in the embryo, were available. We recently established both an imaging approach and a set of markers for the early Arabidopsis embryo. Here, we describe a detailed protocol to use these new tools in imaging cellular reorganization.

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