Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 560987
Title Flow-through real time bacterial biosensor for toxic compounds in water
Author(s) Eltzov, Evgeni; Marks, Robert S.; Voost, Stefan; Wullings, Bart A.; Heringa, Minne B.
Source Sensors and Actuators B: Chemical 142 (2009)1. - ISSN 0925-4005 - p. 11 - 18.
DOI https://doi.org/10.1016/j.snb.2009.08.024
Publication type Refereed Article in a scientific journal
Publication year 2009
Keyword(s) Bioreporter bacteria - Biosensors - Fiber optic - Toxicity
Abstract

A flow-through fiber-optic-based bacterial monitoring system for online monitoring of toxic pollutants in water has been developed. Two bacterial strains containing fusions of recA (DNA damage) and grpE (heat-shock) promoters to the lux operon (CDABE) were immobilized on a fiber optic and tested for their ability to detect pollutants in flowing tap water and surface water. Conditions for running the system for 24 h were optimized and first experiments with the system show (1-h) response times and response heights similar to the previous static systems. Responses were related to the doses and the sensitivity is good (comparable to static systems), but needs to be increased to be able to monitor whether also the low guideline values are exceeded by pollutants. 24-h measurements in tap water demonstrate the ability of the device to run for such a time period, but in river water loss of functionality of the bacteria was observed. This flow-through fiber-optic-based monitoring system has proven to be a useful next step in the development of a simple on-line real time sensor for relevant human toxicants in flowing water.

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