Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 561027
Title Haploid embryogenesis
Author(s) Boutilier, Kimberly; Angenent, Gerrit Cornelis; Soriano Castan, Mercedes; Hui, Li
Source Octrooinummer: WO2015043621, gepubliceerd: 2015-04-02.
Department(s) BIOS Plant Development Systems
Onderzoekschool EPS
Publication year 2015

A switch to haploid embryogenesis is controlled by the activity of histone deacetylases (HDACs). Blocking HDAC activity with HDAC inhibitors (HDACi), e.g. trichostatin A (TSA), inBrassica napus, B. rapa, B. oleracea, Arabidopsis thalianaandCapsicum annuummale gametophytes leads to a large increase in the proportion of cells that undergo embryogenic growth. InB. napus,treatment with one specific HDACi (SAHA) improves the conversion (i.e. germination) of these embryos into seedlings. Existing methods of culturing microspores of angiosperm plants following stress to produce haploid embryos, haploid plants and double haploid plants can be improved by adding HDACi to the culture medium. Advantageously, species hitherto recalcitrant to haploid embryogenesis via microspore culture are rendered useful when using HDACi. Haploid and double haploid plants are of industrial application in the plant breeding programmes.

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