|Title||Supramolecular catch and release : Cyclodextrin-functionalized microfluidics for reversible immobilization of (model) bacteria|
|Author(s)||Willems, Stan B.J.|
|Source||Wageningen University. Promotor(en): A.H. Velders; V. Saggiomo, co-promotor(en): F.W.B. van Leeuwen. - Wageningen : Wageningen University - ISBN 9789463952569 - 162|
|Publication type||Dissertation, internally prepared|
|Availibility||Full text available from 2021-02-07|
The increase in prevalence of pathogenic bacteria coupled provided the motivation for developing and studying platforms for specific on-flow immobilization of bacteria. To approach this goal, we studied the concept of using supramolecular host-guest chemistry for immobilizing model microparticles and bacteria with cyclodextrin functionalized glass surfaces. Fluorescence microscopy was used as the main tool for characterizing surface functionalization and studying surface adhesion interactions.
The experimental chapters explored important steps for developing platforms to study removal of molecules, microparticles and bacteria:
Results show that the cyclodextrin patterned microfluidic devices are versatile tool for studying immobilization of microparticles, such as polystyrene and bacteria, through supramolecular interactions. The retention of particles was specific and selective for cyclodextrin patterns when functionalized with adamantane moieties.