|Title||An automated modular microsystem for enzymatic digestion with gut-on-a-chip applications|
|Author(s)||Haan, P. de; Ianovska, M.A.; Mathwig, K.; Bouwmeester, H.; Verpoorte, E.|
|Source||In: 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. - Chemical and Biological Microsystems Society (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 ) - ISBN 9780692941836 - p. 1593 - 1594.|
|Event||21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017, Savannah, 2017-10-22/2017-10-26|
|Publication type||Contribution in proceedings|
|Keyword(s)||Digestion - Enzyme kinetics - Gut-on-a-chip - Organ-on-a-chip|
Gut-on-a-chip models have gained attention as replacements for other cell-based assays or animal studies in drug development or toxicological studies. These models aim to provide a more accurate representation of the in vivo situation in form and function; however, no digestive processes have been included in these systems so far. This work describes a miniaturized digestive system based on artificial digestive juices that digest liquid samples in a series of three microreactors. After optimization of the pH value of juices and mixtures, samples leading to fluorescent products were digested to demonstrate enzyme functionality and to determine kinetic parameters.